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Margarita Kakava

Evaluation of interactions of magnetic nanoparticles with T-lymphocytes

Iron oxide nanoparticles have broadened novel treatment approaches in cancer therapy due to their ability to function at the cellular and molecular level. According to literature there are various types of iron nanoparticles being used in cancer therapy improving current therapeutic approaches, such as chemotherapy 1 and radiotherapy 2. Based on that, many scientific groups investigate the interactions between iron oxide nanoparticles and different cell lines. Therefore, it is crucial to unravel the cellular internalization mechanism, the biocompatibility and the cellular hyperthermia response. The purpose of this short term scientific mission was to evaluate the cellular interactions of iron oxide nanoparticles, such as biocompatibility and cellular internalization mechanism in human Jurkat cell lines. To accomplish this, viability assays and flow cytometry (FACS) analysis were performed. Two different samples of iron oxide nanoparticles were studied during this period. The toxicity of the nanoparticles was studied by the MTT assay and the flow cytometry technique. The obtained results differed, as the first sample was nontoxic for the studied concentration range, whereas the other one was toxic at higher concentrations. Subsequently, the cellular uptake was studied after 1, 2, 4 and 24_h of incubation in Jurkat cells. The appropriate studied concentrations for each sample were chosen based on their cytotoxic profile. It is worth mentioning that a significant cellular uptake was observed and the uptake percentage increased as a function of incubation time for both samples. Finally, the apoptosis assay was carried out after 1-2_h of incubation for the most toxic sample and about 35% of apoptotic cells were observed.

References

1. Biomaterials Volume 31, Issue 18, June 2010, Pages 4995–5006

2. Theranostics 2015, Vol. 5, Issue 9, 1030-1045